Click the "Select File" button above, and choose your CZI file.
You’ll see a preview, if available.
Click the "Convert file to..." button to extract text information.
Convert CZI to another file type
To convert CZI microscope images to another format, you need ZEN Microscopy Software or other Raster Image software.
Convert a file to CZI
To convert other file formats to the "Microscopy Image Data" file type, you need software like ZEN Microscopy Software or a similar tool.
About CZI files
The .czi file format is a proprietary standard developed by Carl Zeiss Microscopy to store complex, multidimensional image data generated by their microscopes. These files capture highly detailed spatial and temporal information, including Z-stacks, multiple fluorescence channels, time-lapse sequences, and multi-position tiles. Scientists and researchers use this format within the ZEN software ecosystem to analyze biological and material samples.
The main disadvantage of the .czi format is its rigid reliance on specialized software. You cannot open these files natively in Windows, macOS, or standard web browsers. They are often massive in size, frequently exceeding several gigabytes, making them difficult to share with collaborators who do not have a ZEN license or complex open-source tools like ImageJ/Fiji installed. Furthermore, rendering multidimensional data requires heavy processing power.
To make this data accessible, conversion is usually necessary. For scientific archiving and quantitative editing, convert to TIFF or .OME-TIFF to retain the raw pixel values and metadata. If you need to include a specific slice or channel in a presentation or a web publication, convert to PNG or JPG, keeping in mind that you will lose bit-depth, metadata, and multidimensional layers. Drop your file here to view and convert it securely right in your browser.
Convert.Guru analyzes your CZI file, detects the exact format, and lets you read the text inside.
If you want to convert CZI file to JPG, TIFF, PNG, SVS, LSM, AVI, PDF, GIF, BMP, TIF, WEBP or ICO, you can use ZEN Microscopy Software or similar software from the "Scientific Microscope Image Storage" category. In the File menu, look for Save As… or Export….
To convert RAW, PNG, AI, NEF, PSB, DNG, SVG, GIF, EPS, JPG, ARW or PDF files to CZI, try ZEN Microscopy Software or another comparable tool in the "Scientific Microscope Image Storage" category.
The CZI Converter Story
The history of Convert.Guru began over 25 years ago in California with Tom Simondi’s file-format database. A former contributor to Space Shuttle development and a software pioneer of the 1980s, Simondi established a trusted resource for file type analysis that was even referenced by Microsoft Windows XP. Today, we use modern technology to process and convert thousands of file formats while continually improving our CZI converter.