Click the "Select File" button above, and choose your ALI file.
You’ll see a preview, if available.
Click the "Convert file to..." button to extract text information.
Convert ALI to another file type
To convert ALI Alignments to another format, you need MODELLER or other Data software.
Convert a file to ALI
To convert other file formats to the "Bioinformatics Sequence Data" file type, you need software like MODELLER or a similar tool.
About ALI files
.ALI files are predominantly plain text files used in two distinct, high-friction environments: structural bioinformatics and enterprise reporting.
1. Bioinformatics (MODELLER): In scientific workflows, the .ALI format contains protein sequence alignments specifically formatted for MODELLER (used for homology modeling). While human-readable, this format is strict and often incompatible with general-purpose alignment viewers like Jalview or Clustal Omega. Researchers frequently hit a wall when trying to feed an .ALI file into downstream phylogenetic or visualization tools that expect standard FASTA or PHY (Phylip) formats. The conversion challenge involves parsing the specific header lines (structure code, sequence type) that MODELLER requires but other tools reject.
2. SAP ABAP Lists: In the corporate sector, .ALI files appear as exported lists or reports from SAP ERP systems (ABAP List Output). These files essentially lock critical business data into a static text layout. Users typically need to extract this data into XLSX (Excel) for analysis or PDF for archival, rather than dealing with the raw, whitespace-dependent text dump that the .ALI format provides.
Recommendation:
For Bioinformatics: Convert to FASTA for maximum compatibility with web-based tools, or .PIR if migrating to legacy alignment software.
For SAP Data: Convert to CSV or XLSX to make the tabular data usable in spreadsheets.
Convert.Guru analyzes your ALI file, detects the exact format, and lets you read the text inside.
If you want to convert ALI file to CSV, JSON, XML, YAML, YML, TOML, INI, CFG, CONF, DAT, DB or SQL, you can use MODELLER or similar software from the "Bioinformatics Alignment & SAP Reports" category. In the File menu, look for Save As… or Export….
To convert DBF, XML, SQLITE, XLSX, SQL, TSV, ACCDB, YAML, MDB, CSV, ODS or JSON files to ALI, try MODELLER or another comparable tool in the "Bioinformatics Alignment & SAP Reports" category.
The ALI Converter Story
The history of Convert.Guru began over 25 years ago in California with Tom Simondi’s file-format database. A former contributor to Space Shuttle development and a software pioneer of the 1980s, Simondi established a trusted resource for file type analysis that was even referenced by Microsoft Windows XP. Today, we use modern technology to process and convert thousands of file formats while continually improving our ALI converter.